Robert L. Hettich
Senior Research Staff, Scientist

B.S. Chemistry, South Dakota School of Mines and Technology,
    Rapid City, South Dakota

Ph.D. Analytical Chemistry, Purdue University,
   West Lafayette, Indiana

Adjunct Faculty Member, Genome Sciences and Technology (GST) Graduate School, University of Tennessee, Knoxville, Tennessee

Research Interests:

External Collaborations:

Prof. J. Banfield,
    University of California - Berkeley
   

Selected Publications

1. Hettich RL, Stemmler E. Investigation of Oligonucleotide Fragmentation with MALDI-FTMS and Sustained Off-Resonance Irradiation (SORI). Rapid Comm. Mass Spec. (Special ORNL issue), 1996;10:321.

2. Hettich RL Formation and Characterization of Iron-Oligonucleotide Complexes with MALDI-FTICR. J. Amer Soc Mass Spec. (Freiser focus issue), 1999;0:941-49.

3. Hettich RL Investigating the Effect of Transition Metal Ion Oxidation State on Oligodeoxyribonucleotide Binding by MALDI-FTICR-MS. Int J Mass Spec. 2001;204:55-75.

4. Ji HF, Dabestani R, Brown GM, Hettich RL. Synthesis and sensing behavior of cyanoanthracene modified 1,3-alternate calix[4]benzocrown-6: a new class of Cs+ selective optical sensors. Journal of the Chemical Society-Perkin Transactions. 2001;2:585-91.

5. Uchiki T, Hettich RL, Gupta V, Dealwis C. Characterization of Monomeric and Dimeric Forms of Recombinant Sml1p-histag Protein by Electrospray-MS. Anal. Biochem 2002;301:35-48.

6. Kertesz V, Deng H, Asano KG, Hettich, RL, Van Berkel GJ. N-Phenyl-1,4-Phenylenediamine and Benzidine Oxidation Products Identified Using On-Line Electrochemistry/Electrospray Fourier Transform Mass Spectrometry. Electroanalysis. 2002;14:1027-30.

7. VerBerkmoes NC, Strader MB, Smiley D, Howell EE, Hurst GB, Hettich R L, Stephenson, Jr JL. Intact Protein Analysis for Site Directed Mutagenesis Overexpression Products: Plasmid Encoded R67 Dihydrofolate Reductase. Anal Biochem. 2002;305:68-81.

8. VerBerkmoes NC, Bundy JL, Hauser L, Asano KG, Razumovskaya J, Larimer F, Hettich RL, Stephenson, Jr. JL. Integrating "Top-Down" and "Bottom-Up" Mass Spectrometric Approaches for Proteomic Analysis of Shewanella oneidensis. J Proteome Research. 2002;1:239-52.

9. Sharp J, Becker J, Hettich RL. Protein Surface Mapping by Chemical Oxidation: Structural Analysis by Mass Spectrometry. Anal Biochem. 2003;313: 216-25.
10. VerBerkmoes N, Sharp J, Hettich RL. "Mass Spectrometry" in Microbial Functional Genomics, edited by Jizhong Zhou and Dorothea Thompson, John Wiley & Sons, 2004.

11. Keller K, Brodbelt J, Hettich RL, Van Berkel GJ. Comparison of Sustained Off-Resonance Irradiation Collisionally Activated Dissociation and Multipole Storage-Assisted Dissociation for Top-Down Protein Analysis. J Mass Spec. 2004;39:402-11.

12. "Identification of Phosphorylation Sites on the Yeast Ribonucleotide Reductase Inhibitor Sml1," J. Biol. Chem. (2004), 279, 11293-11303.

13. Ross S, Srinivas P, Clifford A, Lee S, Philbert M, Hettich RL. New Technologies for Nutrition Research. J Nutrition. 2004;134:681-85.

14. Gupta V, Peterson C, Dice L, Uchiki T, Racca J, Guo J, Xu Y, Hettich RL, Zhao X, Rothstein R, Dealwis C, Sml1p is a Dimer in Solution: Characterization of Denaturation and Renaturation of Recombinant Sml1p. Biochemistry. In press for summer 2004.

15. Razumovskaya J, Olman V, Xu D, Uberbacher E, VerBerkmoes N, Hettich RL, Xu Y. A Computational Method for Assessing Peptide-Identification Reliability in Tandem Mass Spectrometry Analysis with Sequest. Proteomics. 2004;4:961-9.

16. Strader M, VerBerkmoes N, Tabb D, Connelly H, Barton J, Bruce B, Pelletier D, Davison B, Hettich RL, Larimer F, Hurst G. Characterization of the 70S Ribosome from Rhodopseudomonas palustris Using an Integrated "Top-Down" and "Bottom-Up" Mass Spectrometric Approach. J Proteome Res. In press for summer 2004.

17. Sharp J, Becker J, Hettich RL. Analysis of protein solvent accessible surfaces by photochemical oxidation and mass spectrometry. Anal Chem. 2004;76:672-83.