Abstract
Geobacillus thermoglucosidasius C56-YS93 was one of several thermophilic organisms isolated from Obsidian Hot
Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. Comparison of 16 S
rRNA sequences confirmed the classification of the strain as a G. thermoglucosidasius species. The genome was
sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December
2011 (CP002835). The genome of G. thermoglucosidasius C56-YS93 consists of one circular chromosome of
3,893,306 bp and two circular plasmids of 80,849 and 19,638 bp and an average G + C content of 43.93 %. G.
thermoglucosidasius C56-YS93 possesses a xylan degradation cluster not found in the other G. thermoglucosidasius
sequenced strains. This cluster appears to be related to the xylan degradation cluster found in G. stearothermophilus.
G. thermoglucosidasius C56-YS93 possesses two plasmids not found in the other two strains. One plasmid contains a
novel gene cluster coding for proteins involved in proline degradation and metabolism, the other contains a
collection of mostly hypothetical proteins.