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Correlative fluorescence microscopy and liquid STEM of proteins labeled with quantum dots on intact cells....

by Madeline J Dukes, Diana B Peckys, Niels De Jonge
Publication Type
Journal
Journal Name
ACS Nano
Publication Date
Page Numbers
4110 to 4116
Volume
4
Issue
7

Liquid scanning transmission electron microscopy (liquid STEM) was used to image individual quantum dots bound to receptors in COS7 cells. The same regions on intact fixed cells were imaged both with fluorescence microscopy and liquid STEM. The obtained spatial resolution on was 3 nm. The resolution was sufficient to discriminate between quantum dots and gold nanoparticles for double labeling applications.