Publication Type
Journal
Journal Name
Journal of Proteome Research
Publication Date
Page Numbers
1953 to 1964
Volume
8
Issue
4
Abstract
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol,
or phenol at 5 and 28 °C, both for the wild-type and a mutant strain with mass spectrometry
based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across
all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form
the core proteome. Statistically significant differences were found in the proteomes of cells grown
under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-
degradation pathway was confirmed, but not that for phenol.
