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ORNL’s biosensor system reveals CRISPR activity in poplar plants, which glow bright green under ultraviolet light, compared to normal plants, which appear red. Credit: Guoliang Yuan/ORNL, U.S. Dept. of Energy

Detecting the activity of CRISPR gene editing tools in organisms with the naked eye and an ultraviolet flashlight is now possible using technology developed at ORNL. 

Carrie Eckert

Carrie Eckert applies her skills as a synthetic biologist at ORNL to turn microorganisms into tiny factories that produce a variety of valuable fuels, chemicals and materials for the growing bioeconomy.

Researchers gained new insights into the mechanisms some methane-feeding bacteria called methanotrophs (pictured) use to break down the toxin methylmercury. Credit: Andy Sproles/ORNL, U.S. Dept. of Energy; Jeremy Semrau/Univ. of Michigan

A team led by ORNL and the University of Michigan have discovered that certain bacteria can steal an essential compound from other microbes to break down methane and toxic methylmercury in the environment.

The Oak Ridge National Environmental Research Park encompasses a 20,000 acre area that includes Oak Ridge National Laboratory. Credit: Carlos Jones/ORNL, U.S. Dept. of Energy

Anyone familiar with ORNL knows it’s a hub for world-class science. The nearly 33,000-acre space surrounding the lab is less known, but also unique.

As the leader of ORNL’s Biodiversity and Ecosystem Health Group, environmental scientist Teresa Mathews works to understand the impacts of energy generation on water and solve challenging problems, including mercury pollution. Credit: Carlos Jones/ORNL, U.S. Dept. of Energy

Moving to landlocked Tennessee isn’t an obvious choice for most scientists with new doctorate degrees in coastal oceanography.

ORNL metabolic engineer Adam Guss develops genetic tools to modify microbes that can perform a range of processes needed to create sustainable biofuels and bioproducts. Credit: Carlos Jones/ORNL, U.S. Dept. of Energy

As a metabolic engineer at Oak Ridge National Laboratory, Adam Guss modifies microbes to perform the diverse processes needed to make sustainable biofuels and bioproducts.

ORNL’s Josh Michener, a microbiologist and metabolic engineer, led the discovery of a useful new enzyme that breaks down stubborn bonds in lignin, a polymer found in plants that typically becomes waste during bioconversion. Credit: Carlos Jones/ORNL, U.S. Dept. of Energy

In a step toward increasing the cost-effectiveness of renewable biofuels and bioproducts, scientists at ORNL discovered a microbial enzyme that degrades tough-to-break bonds in lignin, a waste product of biorefineries.

Scientists genetically engineered bacteria for itaconic acid production, creating dynamic controls that separate microbial growth and production phases for increased efficiency and acid yield. Credit: NREL

A research team led by Oak Ridge National Laboratory bioengineered a microbe to efficiently turn waste into itaconic acid, an industrial chemical used in plastics and paints.

A new method uses E. coli to generate DNA with methylation patterns that target microbes recognize and accept as their own, facilitating customization of microbes for biofuels production.

Scientists at the US Department of Energy’s Oak Ridge National Laboratory have demonstrated a method to insert genes into a variety of microorganisms that previously would not accept foreign DNA, with the goal of creating custom microbes to break down plants for bioenergy.

Alex Johs at ORNL's Spallation Neutron Source

Sometimes solutions to the biggest problems can be found in the smallest details. The work of biochemist Alex Johs at Oak Ridge National Laboratory bears this out, as he focuses on understanding protein structures and molecular interactions to resolve complex global problems like the spread of mercury pollution in waterways and the food supply.